The plan:
pipetted & spread 100 uL of 0.1 mM IPTG onto AMP plates, let dry.
Picked a J04450 colony and streaked it on two plates 30 min later. Looking for induction of the RFP in J04450 via IPTG repression of the LacI repressor.
24 hours later, no visible induction :(
Note, spilled ~3 mL of 1M IPTG after sterilizing it. MSDS had no clear clean-up information. Wiped up the spill w/ paper towel and put it w/ autoclave waste. IPTG is heat-sensitive. Washed the table 3x with water and then with bleach.
It would be excellent to have more operable “scram” instructions for situations like this (exactly what to do if a spill or exposure) before working with chemicals. Would like to develop these every time a new chemical is ordered, before it arrives at the lab. Not sure what the best way is to do.
