Went over to the bosslab yesterday (16 May 2011) around 11 PM to check on the plates (18+ hrs of incubation in the “incubator”).
No signs of colonies. Transformation failed, or amp was too conc, or incubator was too hot, or…
SOC broth that was incubated was mildly turbid. The biobrick card used was not sterile, so the organisms were probably not transformed E. coli. Plated 100 uL of the SOC on LB agar plates anyway. We’ll see.




